snpeff
- Repo stars 307
- License NOASSERTION
- Author updated Live
- Author repo SnpEff
- Domain
- Engineering
- Compatible agents
-
- Claude Code
- Cursor
- Cline
- Codex
- Windsurf
- Gemini CLI
- +20
- Trust score
- 94 / 100 · audit passed
- Author / version / license
- @pcingola · NOASSERTION
- Token usage
- Lean
- Setup complexity
- Plug-and-play
- External API key
- Not required
- Operating systems
- Unspecified (assume cross-platform)
- Runtime requirements
- No special requirements
- Permissions
-
- Read-only
- Write / modify
- Network behavior
- Local-only
- Install commands
- 26 variants
Profile is derived at build time from SKILL.md and install vectors. Subject to drift from author intent.
Heads up: 未限定 allowed-tools,默认拥有全部工具权限。
---
name: snpeff
description: Run SnpEff, a variant annotation and effect prediction tool for genomic variants. Use when the u…
category: engineering
runtime: no special runtime
---
# snpeff output preview
## PART A: Task fit
- Use case: Run SnpEff, a variant annotation and effect prediction tool for genomic variants. Use when the user wants to annotate VCF files, build genome databases, or use SnpEff utilities..
- Inputs: target material, constraints, expected output, and acceptance criteria.
- Evidence boundary: follow “Running / Commands / Additional Documentation” and do not present inference as author intent.
## PART B: Execution result
- **01** The card summarizes the use case; runtime output centers on “Run SnpEff, a variant annotation and effect prediction tool for genomic variants. Use when the user wants to annotate VCF files, build genome databases, or use SnpEff utilities.”.
- **02** When the source has headings, the agent prioritizes “Running / Commands / Additional Documentation” so the result follows the author’s structure.
- **03** Typical output includes task judgment, concrete steps, required commands or file edits, validation, and follow-up options.
- **04** Risk context follows the fingerprint: read files, write/modify files; mostly runs locally; usually needs no extra API key.
## Running Rules
- read files, write/modify files; mostly runs locally; usually needs no extra API key.
- Validate with a small sample before expanding scope.
- Return the result, validation criteria, and next iteration options. The source does not require a stable slash command. After installation, invoke the skill by name and describe the task.
Name target files or source material, expected output, forbidden changes, and whether network or shell access is allowed. Permission fingerprint: read files, write/modify files.
Start with a small task and check whether the result follows “Running / Commands / Additional Documentation”. Inspect diffs, logs, previews, or tests before expanding scope.
Confirm the final output includes a concrete result, evidence, and next action. If it stays generic, tighten inputs, boundaries, and acceptance criteria.
---
name: snpeff
description: Run SnpEff, a variant annotation and effect prediction tool for genomic variants. Use when the u…
category: engineering
source: pcingola/SnpEff
---
# snpeff
## When to use
- Run SnpEff, a variant annotation and effect prediction tool for genomic variants. Use when the user wants to annotate…
- Use it when the task has clear inputs, repeatable steps, and validation criteria.
## What to provide
- Target material, scope, expected result, and forbidden changes.
- Whether network, commands, file writes, or external services are allowed.
## Execution rules
- Organize steps around “Running / Commands / Additional Documentation” and keep inference separate from source facts.
- read files, write/modify files; mostly runs locally; usually needs no extra API key.
- Validate with a small sample before expanding the task.
## Output requirements
- Return the deliverable, key evidence, validation method, and next action.
- Mark missing information as unknown; do not invent commands, platforms, or dependencies. The author source anchors workflow facts; repository files anchor sources and commands; Fluxly only adds fit, limitations, and quality judgment.
skill "snpeff" {
input -> user goal + target files + boundaries + acceptance criteria
context -> Running / Commands / Additional Documentation
rules -> SKILL.md triggers / order / output contract
runtime -> no special runtime | read files, write/modify files | mostly runs locally
guardrails -> usually needs no extra API key + small-sample validation + diff/log review
output -> copyable result + checklist + next iteration
} SnpEff
SnpEff is a variant annotation and effect prediction tool. It analyzes VCF files and predicts the functional effects of genetic variants (such as amino acid changes) on known genes.
Full documentation: https://pcingola.github.io/SnpEff/
Running
Use the wrapper script at .claude/skills/snpeff/snpeff.sh. It handles JVM memory defaults and argument passthrough. The JAR file is expected at $HOME/snpEff/snpEff.jar.
IMPORTANT: SnpEff output is typically very large (thousands to millions of lines). ALWAYS redirect output to a file. NEVER let output print to stdout, as it will fill the context window and make the conversation unusable.
# Correct: redirect to file
.claude/skills/snpeff/snpeff.sh <command> [options] [arguments] > output.vcf 2> snpeff.log
# WRONG: never do this
.claude/skills/snpeff/snpeff.sh <command> [options] [arguments]
Commands
| Command | Description | Docs |
|---|---|---|
ann / eff |
Annotate variants (default command) | commandline, running, input/output |
build |
Build a SnpEff database from reference genome files | build_db, build_db_gff_gtf |
buildNextProt |
Build NextProt database from XML files | commands |
cds |
Compare CDS sequences (database check) | commands |
closest |
Annotate closest genomic region | commands |
count |
Count reads/bases overlapping genomic intervals | commands |
databases |
List available databases | commands |
download |
Download a pre-built database | commands |
dump |
Dump database contents (BED/TXT) | commands |
genes2bed |
Create BED file from gene list | commands |
len |
Calculate genomic length per marker type | commands |
pdb |
Build interaction database from PDB/AlphaFold data | build_pdb |
protein |
Compare protein sequences (database check) | commands |
seq |
Translate DNA sequence to protein | commands |
show |
Show gene/transcript text representation | commands |
translocReport |
Create translocation report with SVG | commands |
Additional Documentation
| Topic | File |
|---|---|
| Introduction | introduction |
| Additional annotations | additionalann |
| Building regulation databases | build_reg |
| Cancer samples | cansersamples |
| FAQ | faq |
| Human genomes | human_genomes |
| Integration (GATK, Galaxy) | integration |
| Output summary | outputsummary |
| Troubleshooting | troubleshooting |
| Download & Install | download |
| Examples | examples |
| Help | help |
Decide Fit First
Design Intent
How To Use It
Boundaries And Review